Validation of Spectrophotometer
validation spectrophotometer

Validation of Spectrophotometer

UV-Visible Spectrophotometer is the one of the most important instrument in many laboratories. It is used in the industries, pharmacy labs, and many other places. It plays the major role in the testing of samples and provides the best results.

That’s why it is very important to validate your spectrophotometer working properly or not. You have to validate your Spectrophotometer after a time to check it’s working.

Below Mentioned Some Parameters are Majorly Checked while Validation:

  1. Wavelength Accuracy
  2. Wavelength Repeatability
  3. Baseline Flatness
  4. Absorbance Accuracy
  5. Stray Light

Standards Use for the Validation of the Spectrophotometer

  1. Holmium Oxide Filter
  2. Didymium Filter
  3. Absorbance Spectral Filters
  4. ICUMSA Filter
  5. Photo check (Standards)
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Wavelength Accuracy

Wavelength Accuracy is the parameter which shows the exact wave is coming or not which we set in the instrument.

To validate this we use Holmium Oxide or Didymium Filter, with this we check the closeness between the measured wavelength value and the true wavelength.

Wavelength Repeatability:

It is known as the stability of the wavelength measurement after some time. To check this we use Holmium Oxide or Didymium Filter in the spectrophotometer.

Baseline Flatness

To validate the Baseline Flatness of the Spectrophotometer, we have to scan air in the absorbance Mode and note the highest and lowest deflection in the absorbance unit

Absorbance Accuracy

Use Absorbance Spectral Filters or ICUMSA Filters to validate the Absorbance Accuracy of the Spectrophotometer. If the measured reading will be closer than the True value then the instrument is working fine.